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Mouse Cathepsin S (CTSS) ELISA Kit

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Product Name:

Mouse Cathepsin S (CTSS) ELISA Kit

Cat.#:

JL20724

Regulatory Status:
Alternative:

CTS-S

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.15-10ng/mL
Sensitivity:
0.06ng/mL
Specificity:
This kit recognizes Mouse CTSS in samples. No significant cross-reactivity or interference between Mouse CTSS and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Cancer
  • Mouse Cathepsin S (CTSS) ELISA Kit
  • Mouse Cathepsin S (CTSS) ELISA Kit
  • Mouse Cathepsin S (CTSS) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| CTSS

Cathepsin S is a protein that is encoded by the CTSS gene.Transcript variants utilizing alternative polyadenylation signals exist for this gene.Cathepsin S is a member of the peptidase C1 family of cysteine cathepsins, a lysosomal cysteine protease that may participate in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S can function as an elastase over a broad pH range in alveolar macrophages.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 10 5 2.5 1.25 0.62 0.31 0.15 0
OD 2.36 1.34 0.8 0.48 0.34 0.26 0.22 0.09
Corrected OD 2.27 1.25 0.71 0.39 0.25 0.17 0.13 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Mouse CTSS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 84-101 96
Plasma(n=8) 92-105 102
Tissue Homogenates(n=8) 96-108 105





 

| Linearity

Samples were spiked with high concentrations of Mouse CTSS and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4) Tissue Homogenates(n=4)
1 : 2 Range (%) 84-95 87-96 90-110
Average (%) 90 93 96
1 : 4 Range (%) 89-103 87-108 105-115
Average (%) 94 98 108







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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